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Fig. 3. The addition of laminin to GCPs in suspension activates Src kinase. (A) Western blot of laminin-treated or control GCPs, probed with the indicated antibodies (IB; 
=anti). p85 serves as a loading control. Numbers below the blot are the average ratios for phospho-Src over p85 (arbitrary units normalized to control ± s.e.m.; n=2). (B) Cytoskeleton fractions prepared from GCPs with or without prior laminin treatment (each lane was loaded with the total cytoskeletal preparation from equal GCP samples). Samples were subjected to western blot with the indicated antibodies (IB). The numbers below the blot represent quantitation of Src (arbitrary units normalized to control ± s.e.m.; n=2). Note that SIRP was not detected in the cytoskeleton fraction, but that SHP-2 was present.
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