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Fig. 8. Overexpression of cSIRP affects axonal growth in cortical neurons. (A) The axons of two neurons cultured on laminin were visualized by GFP fluorescence (top) and phase-contrast (middle and bottom). (Left) Neuron transfected with GFP only; (right) neuron transfected with cSIRP plus GFP. The GFP and t=0 phase-contrast images were recorded immediately after the addition of IGF-1. Phase-contrast images were also recorded 1 hour after the addition of IGF-1 (t=1h). The arrows in the images indicate the distance that the growth cons had advanced during 1 hour of observation. Note that the growth cone shown in the left panels advanced much faster than the ones in the right panels. Bar, 10 µm. (B) Quantitation of axon growth rates for neurons cultured on laminin or poly-D-lysine. Data are shown for neurons transfected with GFP only (control), with cSIRP plus GFP, or with mutant cSIRP-FYFF plus GFP. Results are means ± s.e.m. for the number of independent experiments indicated above each bar. Error bars indicate s.e.m. ^*P<0.05 compared to the growth rate measured for control neurons grown on laminin (the left-most bar).

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