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Fig. 3. Immunolocalization of Myo10 in mouse brain. (A-C) Sagittal section from the cerebellum of an adult mouse double-labeled for Myo10 and calbindin, a marker for Purkinje cells. The cell bodies of Purkinje cells (PL) and their dendrites in the molecular layer (ML) are brightly stained by Myo10. Myo10 stains the granule-cell layer (GL) more faintly, and the cerebellar white matter (WM) shows little staining. (D-F) A similar section of the molecular layer at higher magnification shows staining for Myo10 in the dendrite (arrow in E) of a Purkinje cell. (G-I) Sagittal section from the cerebellum of an adult mouse double-labeled for Myo10 and GFAP, a marker for Bergmann glia and other astrocytes. (J-L) A similar section of the molecular layer at higher magnification showing that Myo10 staining is also present in the processes of Bergmann glia (arrow in K). (M-O) Sagittal section from the cerebrum of an adult mouse double-labeled for Myo10 and GFAP showing staining of the ventricular zone (VZ) and subventricular zone (SVZ). Although the parenchyma of the adult mouse cerebrum shows relatively faint Myo10 staining, the ependymal cells lining the ventricle show bright Myo10 staining. The subjacent subventricular astrocytes marked by GFAP staining (red arrow in H) are also Myo10 immunopositive.





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