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Fig. 4. Subcellular localization of endogenous Myo10 in CAD cells. (A) In undifferentiated CAD cells, Myo10 shows a clear localization to the leading edge as well as a more diffuse localization in central cytoplasm. (B) Undifferentiated CAD cells stained with a non-immune control antibody show little or no staining. (C) After ~2 days of serum withdrawal to induce CAD-cell differentiation into a neuronal phenotype, Myo10 staining is observed in cell bodies, neurites, the edges of growth cones and the tips of neuronal filopodia. (D) Differentiated CAD cells stained with a non-immune control antibody show little or no staining. Myo10 antibody no. 3568 was used to stain Myo10 (green) and rhodamine-phalloidin was used to label F-actin (red).





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