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Fig. 7. Puncta of Myo10 can move laterally along the leading edge and fuse with one another. (A) Fluorescence image of an undifferentiated CAD transiently transfected with full-length GFP-Myo10 showing 17 relatively large puncta of GFP-Myo10 (labeled a-q) at the tips of filopodial bundles along the leading edge. (B) Outline of the leading-edge region of this cell that was imaged for 12 minutes and then displayed as a kymograph to highlight movements along the lateral edge. (C) Kymograph illustrating the movements of puncta of Myo10 along the leading edge. Although the larger Myo10 puncta maintained relatively stable positions along the leading edge, smaller Myo10 puncta tended to move laterally along the leading edge until they collided and fused with larger puncta, thus generating a kymograph with a dendritic appearance.





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