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Fig. 4. Representative AFM images of the cytoplasmic face of the nuclear envelope 30 minutes after injecting HSV-1 capsids into X. laevis oocytes. Areas indicated by dashed lines in A are shown at higher magnification in panels C and E. On local patches, the NPC rim is found surrounded by various subviral structures with heights between 20 and 130 nm (B),
15% of which obviously represent capsids (120-130 nm in diameter) (C,D). The remaining viral structures (C,E) exhibit heights between 20 and 120 nm. It is striking that approximately 40% of all viral substructures seen at the cytoplasmic side of the nuclear envelope had a uniform, rod-like shape, with a diameter of 35-40 nm and an estimated length of 130-160 nm or more (F).