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Figure 3


Fig. 3. Capacitation induced changes in tyrosine phosphorylation in wild-type and tFGFR-1 mice. (A) A comparison of tyrosine phosphorylation patterns of wild-type and transgenic, line 4, mouse sperm under capacitating conditions for the times indicated. The relative absorbance of tyrosine phosphorylation for each assay was determined and all data was normalized to ß-tubulin. The relative change in tyrosine phosphorylation was determined and plotted, n=3. (B) A comparison of ERK 1/2 activation in wild-type and transgenic mouse sperm under capacitating conditions for the times indicated. The relative absorbance of ERK phosphorylation for each assay was determined and normalized to the expression of ß-tubulin. The relative changes are plotted, n=3. (C) A comparison of p85 activation in wild-type and transgenic mouse sperm under capacitating conditions for the times indicated. The relative absorbance of p85 phosphorylation for each assay was determined and normalized to ß-tubulin expression. The relative changes in p85 activation are plotted, n=3. *P<0.05; **P<0.01; ***P<0.001.





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