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Fig. 4. Cytochalasin-D-induced conversion of p115HEF1 to p105HEF1 is not due to selective degradation of p115 HEF1. A1-F monolayers on FN-coated dishes were serum-starved overnight and pre-treated with TGF-ß1 (2.5 ng/ml) for 4 hours in the presence of 10 µM lactacystin (LAC) or 50 µM ALLM as indicated. 2 µM cytochalasin D (CD) or equal volumes of carrier solvent DMSO (control) were then directly added into pre-treatment medium, and cells were further incubated for 6 hours. Cell lysates were generated and processed for immunoblotting with anti-HEF1 pAb. Nitrocellulose membranes were stripped and reprobed with anti-p130Cas mAb as loading control.
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