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First published online May 10, 2006

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Spotting developmental regulators

Analysis of patterns of transcription has identified many genes that regulate vertebrate gastrulation, an early morphological event that forms the distinct germ layers of the embryo. But this approach misses developmentally important proteins whose activity is regulated translationally or post-translationally. To identify these, Carl-Philipp Heisenberg's team has used 2D gel electrophoresis and mass spectrometry to compare the patterns of protein synthesis and modification in zebrafish ectodermal and mesendodermal progenitor cells (see p. 2073). Using this comparative proteomics approach, the authors identified 35 differentially produced/modified proteins, most of which were not evident from a parallel transcription analysis. One protein identified by the analysis is ezrin 2, When activated by phosphorylation, this protein links actin filaments to integral plasma membrane proteins, thereby regulating cell shape, adhesion and motility. The authors find that ezrin 2 is activated by phosphorylation in zebrafish mesendodermal cells and is required for proper germ layer morphogenesis. They therefore conclude that comparative proteomics provides a powerful way to identify proteins that regulate early development.

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Identification of regulators of germ layer morphogenesis using proteomics in zebrafish

Vinzenz Link, Lara Carvalho, Irinka Castanon, Petra Stockinger, Andrej Shevchenko, and Carl-Philipp Heisenberg
JCS 2006 119: 2073-2083. [Abstract] [Full Text]  

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content Social Bookmarking                         What's this?