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Figure 3


Fig. 3. Quantification by real-time RT-PCR of mRNA expression of various genes in single oocytes and embryos. Genotype (B6D2F1)-matched unfertilized MII oocytes, 2-cell IVF embryos, 2-cell cumulus clone embryos and 2-cell HSC clone embryos were analyzed. Dppa2, Dppa3, Dppa4, eIF-1A, ERV-L and Hdac1 are considered zygotically activated embryos according to previous studies. Except for Hdac3, values are expressed relative to those in the IVF group (value=1). For Hdac3, the values are expressed relative to Hprt values because of lack of Hdac3 expression in the IVF group. Values with different letters (a and b) differ significantly (P<0.05; Scheffe's F test).





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