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Fig. 2. MT81 and MT81w are anti-mouse CD81 mAbs. Flow cytometry analysis of different cell types stained with the mAbs MT81 (left panels) and MT81w (right panels). Upper panels: Embryonic fibroblasts from wild-type (wt), CD81 knockout (Cd81-/-, thick line) or CD9 knockout (Cd9-/-) mice. The labeling of Cd81-/- cells with mAbs MT81 and MT81w was the same as the control staining. Middle panels: Hepa1-6 cells transfected with a siRNA oligonucleotide targeting CD81 (siCD81, thick line) or with a control siRNA (cont, thin line). Lower panels: HeLa cells transfected with a mouse CD81 cDNA (mCD81, thick line) or with the vector alone (mock, thin line). The thin gray line represents the control staining.