spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 4


Fig. 4. MT81w only binds to CD81 associated with tetraspanins. (A) Hepa1-6 cells were lysed in the presence of CHAPS, Brij 97, digitonin or Triton X-100 before immunoprecipitation with the anti-CD81 mAbs MT81 and MT81w or with the anti-CD9 mAb 4.1F12. Immunoprecipitates were analyzed by western blotting using biotin-labeled anti-CD81 (MT81) and anti-CD9 (4.1F12) mAbs. Note that MT81w precipitates both CD81 and CD9 in CHAPS and Brij 97, but not in digitonin or Triton X-100. (B) Hepa1-6 cells were lysed in 1% Brij 97 before five rounds of immunoprecipitation with anti-CD9 mAb 4.1F12 to deplete CD9 (depl. +) or with beads alone as a control (depl. -). The supernatants were then used for immunoprecipitations as in A. The membranes in each panel were given the same exposure.





Right arrow Return to article