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Figure 6


Fig. 6. CD81 localization in tetraspanin-enriched microdomains depends on cholesterol. (A) Hepa1-6 cell monolayers were treated with 10 mM MßCD for 15 minutes at 37°C (or left untreated as a control) before fixation and double labeling with anti-CD9 mAb 4.1F12 (right panel) and anti-CD81 mAbs MT81 or MT81w (left panel). Bars, 40 µm. (B) Flow cytometry analysis of Hepa1-6 cells stained with the anti-CD81 mAbs MT81 and MT81w or the anti-CD9 mAb 4.1F12. Upper panels: cells were treated with MßCD (thick black line) or left untreated (thin black line). Middle panels: cells were treated with MßCD (thin black line) followed by MßCD/cholesterol (thick black line). Lower panels: cells were treated with MßCD/cholesterol (thick black line) or left untreated (thin black line).





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