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Figure 9


Fig. 9. Host cholesterol is required for P. yoelii sporozoite invasion. (A-C) Hepa1-6 cells were treated with MßCD followed by MßCD/cholesterol when indicated, before infection by P. yoelii sporozoites and quantification of exo-erythrocytic forms (EEFs) at 24 hours (A) or intracellular sporozoites at 3 hours (B). (C) Cells were treated either before (black bars) or 3 hours after (open bars) infection by sporozoites. (D) Primary mouse hepatocytes were treated with MßCD followed by MßCD/cholesterol when indicated before infection with P. yoelii sporozoites. The number of infected cells was determined as described in the Materials and Methods section. **P<0.01, as compared to untreated control.





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