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Figure 8


Fig. 8. The distal portion of the C-terminal tail is not necessary for lysosomal targeting of OA1 in HeLa cells. (A) Immunofluorescence analysis of HeLa cells transiently transfected with an expression vector for mycHis-tagged OA1CT2, which lacks the 65 C-terminal amino acids of CT. Exogenous OA1 was immunodecorated with anti-His6 antibodies and lysosomes were labeled using the fluid-phase marker Lucifer Yellow at 1 hour chase prior to fixation (L.Y.). In the merge, areas of colocalization can be observed in yellow/orange. Bar, 10 µm. (B) Quantitative immunofluorescence analysis of the subcellular distribution displayed by OA1CT2 constructs in transiently transfected HeLa cells. Following immunodecoration with anti-His6 antibodies, transfected cells were counted and classified as described in Fig. 5A. Results represent the mean ± s.e.m. of 3-8 independent experiments. The total number of cells counted is indicated.





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