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Figure 9


Fig. 9. The distal portion of the C-terminal tail is not necessary for lysosomal/melanosomal targeting of OA1 in MNT1 cells. (A) Quantitative immunofluorescence analysis of the colocalization between exogenous mycHis-tagged OA1CT2 and markers of various intracellular compartments in transiently transfected MNT1 cells. Cells were double labeled with anti-His6 antibodies and with antibodies to OA1 (that recognize the endogenous protein, but not OA1CT2), LAMP1, Pmel17, EEA1, or with Lucifer Yellow at 1 hour chase. Results are expressed as the percentage of double-positive vesicles out of all His6-positive compartments per cell and represent the mean ± s.d. of the data pooled from 1-6 independent experiments. The number of vesicles and cells counted is indicated. (B) Quantitative immunofluorescence analysis of the subcellular distribution displayed by OA1CT2 constructs in transiently transfected MNT1 cells. Following immunodecoration with anti-His6 antibodies, transfected cells were counted and classified as described in Fig. 5A. Results represent the mean ± s.e.m. of 3-10 independent experiments. The total number of cells counted is indicated.





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