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Figure 2


Fig. 2. Rac1 must activate two pathways to induce hemocytes activation. (A-F) Hemocyte actin cytoskeleton was visualized using TRITC-phalloidin (red), the nucleus was stained with DAPI (blue) (A) He-Gal4 (B) UAS-Rac1; He-Gal4 (C) UAS-Rac1F37A; He-Gal4, hemocytes were also stained with anti-phosphorylated-tyrosine antibody (green) (D) UAS-Rac1Y40C; He-Gal4 (E) UAS-Rac1F37A; UAS-Rac1Y40C; He-Gal4 (F) UAS-Rac1N17/He-Gal4. (G) F-actin expression levels of the various Rac1 alleles. He-Gal4 was crossed with different Rac1 alleles and hemocytes were bled from wandering third-instar larvae. The hemocytes were stained with TRITC-phalloidin. Imagetrak was used to measure fluorescence intensity of at least 100 hemocytes from three different larvae. Different letters indicate similar groups (i.e. `a' is significantly different from `b' or `c' and so on; Student's t-test, P<0.01). (H) Determination of plasmatocyte diameter. The cell diameter of plasmatocytes from the various genotypes was measured, as described in Materials and Methods, and the diameter (µm) for 25 hemocytes was plotted. *, significant difference (Student's t-test, P<0.01) compared with the parental UAS and He-Gal4 strains.





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