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Figure 3


Fig. 3. Reducing the amounts of cofilin partially rescues Rac1Y40C. (A-E) Hemocytes were bled from early third-instar larvae and stained with TRITC-phalloidin (red), the nucleus was stained with DAPI (blue). (A) UAS-Rac1;He-Gal4 (B) UAS-Rac1Y40C; He-Gal4 (C) y[1] w[67c23]; P{w[+mC]=lacW}tsr[k05633]/CyO, Kr-Gal4, UAS-GFP (D) UAS-Rac1Y40C/P{w[+mC]=lacW}tsr[k05633]; He-Gal4 (E) He-Gal4. (F) F-actin expression levels of different Rac1 alleles crossed to He-Gal4 and y[1] w[67c23]; P{w[+mC]=lacW}tsr[k05633]/CyO, Kr-Gal4, UAS-GFP. Hemocytes were bled from wandering third-instar larvae and stained with TRITC-phalloidin. Imagetrak was used to measure fluorescence intensity of at least 100 hemocytes from three different larvae. *, significant difference (Student's t-test, P<0.01) compared with the parental UAS and He-Gal4 strains. (G) Hemocyte counts after overexpression of various UAS alleles. Hemocytes were counted from at least 15 individual larvae. *, significant difference (Student's t-test, P<0.01) compared with the parental UAS and He-Gal4 strains. (H) Determination of plasmatocyte size. The cell diameter of plasmatocytes from the various genotypes was measured, as described in Materials and Methods, and the diameter (µm) for 25 hemocytes was plotted.





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