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Figure 6


Fig. 6. Loss of Rac1 or Bsk does not block lamellocyte activation after parasitization. Hemocytes were recovered from larvae 40 hours after parasitization by the parasitoid L. boulardi G486. (A) Lamellocytes were recovered from parasitized Hml{Delta}-Gal4 control, UAS-BskIR/Hml{Delta}-Gal4 or, homogygous Rac1J11 loss-of-function larvae and stained with the L1 lamellocyte-specific antibody (green), the nucleus was stained with DAPI (blue). (B) Lamellocytes recovered from parasitized Hml{Delta}-Gal4 control, UAS-BskIR/Hml{Delta}-Gal4, or homogygous Rac1J11 loss-of-function larvae and stained with anti-phosphorylated-FAK antibody (red), anti-phosphorylated-tyrosine antibody (green) and Alexa-Fluor-350-phalloidin (blue). In the merged pictures, overlap of the three stainings is light violet (arrows). (C) Encapsulation of wasp eggs in parasitized Hml{Delta}-Gal4 control UAS-BskIR/Hml{Delta}-Gal4 larvae or homogygous Rac1J11 loss-of-function larvae. Numerical values for proper encapsulation percentages [(Number of properly melanized wasp eggs/number of parasitized larvae) x 100] are presented above each bar. Numbers indicate the number of wasp-parasitized larvae. Numbers in parentheses indicate percentage of total larvae with a properly melanized wasp egg.





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