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Figure 7


Fig. 7. Tau protein specifically interacts with {gamma} or {alpha}-satellite DNA sequences. (A) 400 ng purified tau protein were incubated with 32P-labeled {gamma}-satellite DNA probe in the absence (lane 2) or presence of an excess of unlabeled fECFD DNA fragment (lanes 3,4), unlabeled sonicated poly dI/dC (lanes 5-7), unlabeled sonicated poly dG/dC (lanes 8-10) or unlabeled {gamma}-satellite DNA (lanes 11,12). (B) 400 ng purified tau protein were incubated with 32P-labeled {alpha}-satellite DNA probe in the absence (lane 2) or presence of an excess of unlabeled fECFD DNA fragment (lanes 3,4), unlabeled sonicated poly dI/dC (lanes 5-7), unlabeled sonicated poly dG/dC (lanes 8-10) or unlabeled {alpha}-satellite DNA (lanes 11,12). The arrows indicate the shift observed for the complexes between the satellite probe and tau and the free-labeled probe at the bottom of the gel. The EMSA gels were dried and radioactivity was visualized using PhosphorImager and the computer program ImageQuant.





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