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Figure 1


Fig. 1. (A) Deduced protein sequence of mouse KIFC5A, isolated from the hippocampus of embryonic stage E13. The motor domain is located at the C-terminal. Underlined sequences are conserved motor domain `signature motifs', including the ATP-binding site (GX4GKT). The sequence to which an anti-peptide antiserum was produced is boxed. Shading shows the sequence of recombinant KIFC5A-s, used for motility and protein interaction assays. (B) Predicted probability of coiled-coil formation and protein domains in KIFC5A. Prediction of coils was based on the algorithm of Lupas et al. (Lupas et al., 1991) and domain analysis was performed using the Predict Protein Server (Rost and Liu, 2003). A sketch of KIFC5A overall organisation is shown at the bottom of the panel. (C) Detection of KIFC5A in NIH 3T3 cells. Western blot analysis of a total protein extract from NIH 3T3 fibroblasts, using the affinity-purified anti-peptide antibody to KIFC5A, reveals a band consistent with the predicted Mr of 69.253x103.





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