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Figure 2


Fig. 2. Expression of KIFC5A in the brain is subject to developmental and cell-type-specific regulation. (A) Comparative analysis of levels of KIFC5A cDNA (oligo set 3), synthesised from identical amounts of RNA isolated from the hippocampus of E13 and E18 mice, juvenile (2.5 weeks) and adult (3 months) animals, as well as from pure, proliferating, glia astrocyte cultures (newborn animals) and NIH 3T3 fibroblasts. Equivalent reactions for GAPDH (oligo set 4) were used as internal standards (bottom panel). (B) Tissue-specific pattern of expression of KIFC5A. Equivalent RT-PCR reactions from various mouse tissues (Str., striated). A mock reverse transcription reaction with E13 RNA was the negative (neg.) control. Equivalent reactions for L19 (oligo set 5) were used as internal standards (bottom panel).





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