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Figure 3


Fig. 3. (A) In vitro motility assay of fluorescently labelled MTs in the presence of the affinity-purified recombinant truncated protein KIFC5A-s. Movement of MTs on coverslips coated with KIFC5A-s was studied. The arrow points to a gliding MT. MTs moved at a maximum speed of 1.26 µm minute-1, n=40 (95% confidence). (B) Movement of polarity-marked MTs on recombinant KIFC5A-s. The minus-end of MTs is more brightly labelled. The white bar is at a stable position in all panels. The MT shown glided towards its plus-end, indicating minus-end-directed motility of KIFC5A. Video images were taken at the indicated time intervals (s, seconds). Bar, 10 µm.





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