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Fig. 1. Oxygen consumption of day 4 blastocysts cultured in KSOM or KSOMaa with or without 10 µM NONOate and/or 1 mM L-NAME. The number of determinations is shown in parentheses. Values are mean ± s.e.m. Significant differences in oxygen consumption in the various solutions were observed compared with levels in the relevant control (***P<0.001) and between the two control cultures in KSOM and KSOMaa (aP<0.001).