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Figure 3


Fig. 3. A two-cell mouse embryo stained with DAF-FM and exposed to 10 µM digitonin to permeabilise cell and nuclear membranes. Images show DAF-FM staining (a) before, (b) after 1 minute, and (c) after 2 minutes of digitonin treatment. The punctate pattern revealed after release of cytoplasmic and nuclear DAF-FM into surrounding media appears to colocalise with mitochondria in the embryo stained with both DAF-FM and Mitotracker Deep Red (c,d,e). Bars, 20 µm.





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