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Figure 4


Fig. 4. Example of a FRET experiment. Two-cell mouse embryos were dual stained with DAF-FM and Mitotracker Deep Red. Images are those taken before (a,c) and after (b,d) photobleaching of the area indicated with the yellow circle. DAF-FM (a,b) and Mitotracker Deep Red (c,d) are separated for clarity. An increase in DAF-FM fluorescence is detected after photobleaching the selected area. Bars, 5 µm.





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