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Figure 5


Fig. 5. GABAB receptors colocalize with the clathrin-adaptor protein AP2. (a) Cells labeled with an anti-GABAB receptor antibody (red) and an anti-adaptin ß antibody (green). Colocalization of GABAB receptors and AP2 proteins is seen as a yellow fluorescence (image acquired at cell-surface level). Bar, 10 µm. (b) 2D cytofluorogram. Colocalized pixels are visualized in blue. (c,d) Colocalization of GABAB receptors (15 nm gold particles) and ß2 adaptin (10 nm gold particles) in electron microscopy. (d) Proteins from Paramecium primaurelia (line 1) and Jurkat cells (line 2) were subjected to SDS-PAGE and western blotting. A protein band with an estimated molecular mass of 105 kDa was detected with an anti-adaptin ß antibody. The position of the molecular mass marker is shown on the right. Bars, 100 nm.





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