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Fig. 2. 2D gel electrophoresis. Three Cy-dye labeled extracts (50 µg protein each) were combined and separated by 2D gel electrophoresis. First dimension: 24 cm strips pI 4-7 (A) and pI 7-11 (B). Second dimension, 25.5 x 20.5 cm 10% SDS polyacrylamide gels. Spots differentially regulated between ectodermal and mesendodermal cells are outlined in red, numbered and listed in Table 1.