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Figure 6


Fig. 6. Ezrin2 morpholino antisense oligonucleotides efficiently reduce ezrin2 protein level and impair gastrulation. (A) Placement of the ezrin2 MOs used in this study (MO1 and MO2) on the ezrin2 mRNA. MO1 targets the translation initiation site and MO2 is placed in the upstream 5'UTR of ezrin2 mRNA. (B) Injection of ezrin2 MO1 efficiently reduces ezrin2 protein as detected by western blotting with ERM antibody (tubulin antibody was used as loading control). Co-injection of 100 pg ezrin2 mRNA with 8 ng of ezrin2 MO1 restores ezrin2 protein expression. (C) Quantification of the early loss-of-function phenotypes and partial rescue by ezrin2 mRNA injection. One-cell-stage embryos were injected with morpholino MO1 or MO2 and scored for blastoderm detachment or epiboly defects (wt 0/122, 2 ng MO1 37/91, 4 ng MO1 53/81, 4 ng MO2 38/109, 8 ng MO2 42/89). To rescue ezrin2 morpholino-induced phenotypes 4 ng ezrin2 MO2 followed by 100 pg ezrin2 mRNA were injected. Embryos were scored for blastoderm detachment or epiboly defects (MO2 20/107; MO2 + ezrin2 mRNA 9/90).





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