JCS02915 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Quantitative analysis of TJ profile length. En face images were skeletonized using Metamorph 6 (A,B) and actual linear junction length between two adjacent tricellular zones was determined using the fiber length measuring tool (C). A straight line connecting the two tricellular zones was then drawn and hinged at up to two sites to maximize alignment over the junction (D). The length of the hinged line was also measured. See Materials and Methods for further details.