spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. Knockdown of mLgl increases interaction of aPKC-PAR-6 with PAR-3 or Cdc42. (A) Confluent monolayer of control cells (21-1 or 21-7) or mLgl kd cells (24-15 or 24-16) was lysed with lysis buffer and clarified by centrifugation. The supernatant fraction (Sup, lower panels) containing total 1 mg protein was subjected to immunoprecipitation with anti-PAR-6ß antibody and precipitated proteins were analyzed by western blot (IP, upper panels). Three independent experiments were performed for each clone and numbered as 1, 2 and 3. Notice that the co-precipitation of PAR-3 or Cdc42 with PAR-6 was increased in mLgl kd cells. (B-D) Quantification of the amount of PAR-3 (B), aPKC{lambda} (C) or PAR-6ß in IP fraction. The amount of PAR-3 increased significantly in both mLgl kd cells (*P<0.005, **P<0.001), whereas the amount of aPKC{lambda} or PAR-6ß was not changed in these cells.





Right arrow Return to article