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Figure 5


Fig. 5. Knockdown of PAR-3 accelerates disassembly of apical proteins during depolarization of MDCK cells. Confluent monolayers were incubated with gp135 mouse antibody for 1 hour. After washing out the antibody, the cells were incubated with LC medium for the indicated time. The cells were fixed, permeabilized and stained with Alexa Fluor 488-conjugated anti-mouse IgG together with rhodamine-phalloidin. (A) Projected views of optical sections from the apical to the basal membranes of cells. (B) Confocal z-sections. Arrowheads indicate the cells with intracellular gp135 and F-actin staining. (C) Quantification of cells with aggregated gp135 staining after incubation with LC medium for 5 hour. The percentage of total cells is shown. Values are mean (±s.d.) of three independent experiments. Bars, 20 µm.





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