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Fig. 7. Knockdown of mLgl inhibits re-establishment of intercellular apical domains induced by collagen-gel overlay whereas knockdown of PAR-3 accelerates it. (A) mLgl kd cells (24-15 and 24-16) or control cells (21-1 or 21-7) were cultured on collagen-coated glass for 24 hours, after which the cells were overlaid with collagen gel for 48 hours. The cells were then fixed and triple stained with anti-gp135, anti-ZO-1 and anti-E-cadherin antibodies. (B) PAR-3 kd cells (13-32) or control cells (11-10) were cultured on collagen-coated glass for 24 hours, after which the cells were overlaid with collagen gel for 10.5 or 24 hours. The cells were fixed and double stained with anti-gp135 and anti-ZO-1 antibodies. (C) Quantification of colonies with concentrated gp135 staining at intercellular regions at indicated times after collagen-gel overlay. Scale bars represent 20 µm. Photographs represent the projected views of confocal sections. Bars, 20 µm (A) or 50 µm (B).
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