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Fig. 8. Knockdown of mLgl mislocalizes apical gp135 to outer surface of cyst where cells face collagen gel at initial stage of cystogenesis. mLgl kd cells (#1) or control cells were suspended in collagen gel and cultured for 2 days. Then, cysts were fixed and stained with anti-gp135 and anti-ß1 integrin antibodies. The respective major examples are shown. Notice that gp135 staining was detected at the outer surface of cell aggregates derived from Lgl kd cells, whereas gp135 staining was detected at the inner surface of cell aggregates from control cells. Each photograph represents the single x-y confocal section. Bars, 20 µm.
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