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Fig. 2. Requirement for PI 3-kinase signaling in PKB and MAPK activation differs for T. gondii infection and LPS stimulation. MØs were preincubated 2 hours with PI 3-kinase inhibitor wortmannin (WM, 50 ng/ml) or solvent (DMSO) alone (0), then infected with T. gondii (6:1 ratio of parasites to cells; >90% infected) or stimulated with LPS (100 ng/ml). At the indicated time points, cells were collected for western blot analysis for total and phosphorylated forms of each signaling intermediate. This experiment was performed three times with the same result.