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Figure 3


Fig. 3. Signaling mediated by Gi protein-coupled receptors is required for Toxoplasma-induced PKB and ERK1/2, but not p38 MAPK, activation. MØs were set up as described in Fig. 2, after 2 hours preincubation with pertussis toxin (PTx, 50 ng/ml) or solvent (H2O) alone (0) followed by infection with T. gondii (6:1 ratio of parasites to cells) or stimulation with LPS (100 ng/ml). At the indicated time points, cells were collected for western blot analysis measuring total and phosphorylated form of each signaling intermediate. This experiment was repeated three times with essentially identical results.





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