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Figure 8


Fig. 8. Lack of Cy2-RAP uptake in megalin/lrp2 morphants and of 70kDa-FD in dab2 morphants. Confocal fluorescence microscopy on (A) whole mounts and (B) transversal sections through the pronephic ducts of 72 hpf wild-type and megalin/lrp2 morphants injected with (A) Cy2-RAP or (B) dab2 morphants injected with 70kDa-FD. megalin/lrp2 morphants fail to clear Cy2-RAP from the pronephric duct (A). Loss of renal uptake of 70kDa-FD despite correctly localized megalin/LRP2 at the apical membrane of dab2 morphants (B). Bars, 100 µm (A) and 5 µm (B).





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