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Fig. 1. Domains of JP-45 used to identify the Cav1.1 binding sites. (A) Schematic representation. (B) 10% SDS-PAGE and Coomassie Brilliant Blue staining of the GST-JP-45 fusion proteins purified by glutathione-Sepharose. The numbers above each lane indicate the aa residues that were fused in frame in the pGex plasmid to yield the GST-JP-45 fusion protein.