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Figure 6


Fig. 6. Overexpression of JP-45 in C2C12 cells does not affect the expression levels of the Cav1.1 subunit. C2C12 cells were transfected either with the pFP-N3-DsRed2-JP-45 vector or with pFP-N3-DsRed2 alone as control. (A) Microsomes were prepared from transfected differentiated C2C12; 5 µg protein were separated on a 10% SDS-PAGE, blotted onto nitrocellulose and probed with anti-JP-45 polyclonal Ab, followed by HR-coupled protein G. The immunoreactive band was visualized by chemiluminescence. Note that control cells show the endogenous JP-45 immunoreactive band alone, whereas cells transfected with pFP-N3-DsRed2-JP-45 show an additional band of approximately 70 kDa, representing the DsRed-JP-45 fusion protein. (B) Immunoprecipitation and western blot analysis of Cav1.1 subunit expression in C2C12 cells transfected with pFP-N3-DsRed2 or pFP-N3-DsRed2-JP-45. Results are representative of three different transfection experiments.





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