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Figure 9


Fig. 9. JP-45 gene silencing modifies Cav1.1 charge movement. Charge-movement versus membrane-voltage (Vm) recorded in C2C12 cells transfected with JP-45 siRNA (n=18). Control cells were transfected with pSHAG vector (n=17). (A) Data points, expressed as mean ± s.e.m., were fitted to a Boltzmann equation (see text). Best fitting parameters are shown in Table 2. (B,C) Charge-movement records in the -30 mV to +30 mV range. Numbers on the left indicate the membrane potential. Dotted lines represent the baseline. (D,E) Immunoprecipitation and western blot analysis of Cav1.1 expression in C2C12 cells transfected with siRNA JP-45 and control pSHAG vector. Two assays from a total of four. The Cav1.1 expression in D and E decreased by 69% and 28%, respectively. The location of the molecular mass standards in kDa are depicted on the right.





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