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Figure 1


Fig. 1. Morphology of the ERGIC. (A) Ultrastructure of ERGIC clusters (circled). Immunogold-labeled ultrathin cryosection of a HepG2 cell showing the subcellular distribution of the ERGIC marker ERGIC-53 (10 nm gold) and the rough ER marker protein disulfide isomerase (5 nm gold). Modified and reprinted with permission from Klumperman et al. (Klumperman et al., 1998). (B) Close apposition of ERGIC clusters and ER-exit sites (ERES) visualized by confocal double immunofluorescence microscopy. HeLa cells were fixed, permeabilized and stained for the ERGIC marker ERGIC-53 and the ERES marker Sec31, a subunit of COPII (anti-Sec31 was kindly provided by Wanjin Hong, Institute of Molecular and Cell Biology, Singapore). Note that the majority of ERGIC-clusters are localized close to ERES. The enlargement of the boxed area (right-hand panel) shows partial overlap of ERGIC-53 and Sec31. Bar, 5 µm. (Micrograph kindly provided by Houchaima Ben-Tekaya, University of Basel, Basel, Switzerland.)





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