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Figure 6


Fig. 6. Increased expression of eMyHC in mdx/{alpha}7-/- mice. (A) Immunofluorescence was used to detect eMyHC expression in tibialis anterior muscle fibers from 3-week-old mice. Muscle from wild-type, mdx and {alpha}7-/- mice showed few eMyHC-positive fibers (green). Many muscle fibers from mdx/{alpha}7-/- mice were positive for eMyHC. Rhodamine-labeled WGA (red) was used to define muscle fibers. Bar, 10 µm. (B) Quantitation of eMyHC-positive fibers. Few eMyHC-positive muscle fibers were counted in wild-type, mdx or {alpha}7-/- mice. By contrast, an average of 7.46 fibers per field were positive for eMyHC in mdx/{alpha}7-/- mice, which is significantly different from the controls (*P<0.05).





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