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Figure 2


Fig. 2. Expression of Rab3A, Rab27A and Rab33A in PC12 cells. The same amount of recombinant FLAG-tagged Rab3A, Rab27A, Rab33A, Rab33B or Rab37 expressed in COS-7 cells (lanes 1-5) and total homogenates of PC12 cells (20 µg, lane 6) were loaded on 10% SDS-PAGE and immunoblotted with HRP-conjugated anti-FLAG (bottom panel), anti-Rab3A-, anti-Rab27A-, anti-Rab33A-, or anti-Rab37-specific antibody. Note that each of the four antibodies specifically recognized a single Rab isoform, and that Rab3A, Rab27A and Rab33A are endogenously expressed in PC12 cells. The positions of the molecular mass markers (x10-3) are shown on the left.





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