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Figure 8


Fig. 8. Comparison of furrow ingression and cleavage in wild type and mutant vibrator primary spermatocytes. (A,B) Selected images from a time-lapse series of wild-type (Fig. 6, panel A) and vibS110416 / Df(3R)Dl-BX12 (Fig. 7, panel B) primary spermatocytes. (C) Graphic shows measurements of furrow ingression (distance on one focal plane between opposing furrows observed by DIC) and width of the central spindle (cs) (measured from fluorescence of GFP-labelled microtubules). The vibrator mutant shows a delayed furrow initiation after anaphase onset, a slower rate of furrow ingression and a sudden relaxation of the ingressing membrane (52 minutes in B and C). Furthermore, its central spindle does not become tightly compacted when compared with the wild-type spermatocyte. Successful cytokinesis occurred in the control cell at 28 minutes (A, see blue curve in C). Anaphase onset occurred at zero minutes in C. Time in minutes. Bar, 10 µm.





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