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Figure 5


Fig. 5. MORN1 interacts with the cytoskeleton. Parasites were extracted using different detergents and separated into pellet (P) and soluble (S) fraction. Controls consist of intact parasites (PBS, all protein in pellet) and parasites solubilized by boiling in 1% SDS (all proteins in supernatant). (A) Extracts were subjected to SDS-PAGE, blotted and probed with antibodies. Cytoplasmic YFP-YFP (Gubbels et al., 2003) served as fully soluble control. (B) MORN1 signals were quantified and expressed as percentages of the pellet plus supernatant sum. (C-F) Parasites expressing Myc-tagged myosin C were labeled with antibodies to MORN1 (red, C) and Myc epitope (green, D). A vacuole containing four dividing parasites is shown; note posterior division rings (indicated by arrows and in inset) labeled with both antibodies.





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