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Figure 7


Fig. 7. SiRNA to ERK2 causes a reduction in the amount of cytosolic lipid droplets. (A) Immunoblots of ERK2 as well as of GRP78 and GAPDH in cells transfected with ERK2 siRNA, or with control siRNA. The cells were transfected with the constructs 2 days before the experiment. (B,C) Effect of ERK2 siRNA or a control siRNA on lipid droplet formation in cells treated with oleic acid. The cells were transfected 2 days before the experiments and treated with oleic acid for 2 hours before being stained with Oil Red O. (B) Micrographs showing staining. Bar, 10 µm. (C) BioPix software was used to calculate the total area of red pixels in Oil Red O-stained droplets per cell after transfection with ERK2 siRNA or control siRNA. Values are mean ± s.d. of all cells in 20 randomly selected micrographs from each group (P=0.001, t-test). (D) Influence of ERK2 siRNA on the amount of triglycerides in the cell. The cells were treated as in B, and triglycerides were determined as in Fig. 4 (mean ± s.d.; cells from six different pairs of transfected culture dishes; P=0.010, t-test).





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