JCS02926 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1A
• Supplemental Figure 1B -

  Fig. S1. LA disrupts the actin cytoskeleton and induces activation of caspase-3 in adherent MCF-10A and MCF-10A Ras cells. (A) Cells were grown on coverslips and treated with 5 μM LA for 16 hours. Cells were then fixed with 3% paraformaldehyde, permeabilized and stained with Topro-3 anti caspase-3 (Cell Signaling), anti-actin (Rhodamine phalloidin). (B) Western blot probing pMLC20 of MCF-10A and MCF-10a Ras cells treated with ML-7, ML-9, and Y-27632.

• Supplemental Figure 2A
• Supplemental Figure 2B -

  Fig. S2. Total cell surface expression of β₁ integrin in MCF10A cells after treatment with myosin II, MLCK and actin polymerization inhibitors for 2 hours. (A,B) Normal and Ras-transformed MCF-10A cells were grown on coverslips, treated with inhibitors for 2 hours, and stained with a β₁ integrin antibody, P5D2, to detect total levels of β₁ integrin.