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Figure 1


Fig. 1. FACS analysis of cells treated with MLCK and ROCK inhibitors. (A) Percentage apoptosis of untransformed and Ras-transformed MCF-10A cells grown on polyHEMA or treated with inhibitors of MLCK or ROCK. Cells were plated on polyHEMA and adherent cells were treated with ML-7 (40 µM), ML-9 (40 µM) and Y-27632 (30 µM) for 16 hours. Cells were then collected, stained with annexin V FITC and propidium iodide and subjected to FACS analysis. Results presented are the mean ± s.e.m. of at least three experiments. (B) Percentage apoptosis of MDCK and MDCK Ras cells grown on polyHEMA or treated with inhibitors of MLCK or ROCK. (C) Example plot of untreated MCF-10A cells, and MCF-10A cells treated with ML-7.





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