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Figure 3


Fig. 3. Inhibition of MLCK by ML-9 induces activation of caspase-3 in adherent MCF-10A and MCF-10A Ras-transformed cells. Cells were grown on coverslips and treated with ML-9 (40 µM) for 16 hours. Cells were then fixed with 3% paraformaldehyde, permeabilized and stained with Topro-3, anti caspase-3 (Cell Signaling) and anti-actin (Rhodamine-phalloidin).





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