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Figure 4


Fig. 4. Inhibition of ROCK by Y-27632 does not activate caspase-3 in adherent MCF-10A and MCF-10A Ras-transformed cells. Cells were grown on coverslips and treated with Y27632 (30 µM), for 16 hours. Cells were then fixed with 3% paraformaldehyde, permeabilized and stained with Topro-3, anti caspase-3 (Cell Signaling) and anti-actin (Rhodamine-phalloidin).





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